mouse anti sv2 igg antibody  (Developmental Studies Hybridoma Bank)

Journal: STAR Protocols

Article Title: Protocol for applying expansion microscopy to the study of mammalian neuromuscular junctions

doi: 10.1016/j.xpro.2025.104272

Figure Lengend Snippet: Data analysis methods of expanded NMJs (A) Example of an expanded human NMJ stained with α-Bungarotoxin to label AChRs (cyan) and immunolabeled for SV2 to visualize presynaptic vesicles (magenta). Scale bar= 4 μm. A zoomed-in region (i) illustrates the selected area for measurement. AChR width was quantified on thresholded images (ii), indicated by red bars. AChR strip length and inter-strip spacing were measured on skeletonized images (iii), with yellow bars showing strip length and blue bars indicating spacing. Scale bar in (i)= 2 μm. (B) Mouse NMJ labeled as in (A). The yellow-dotted rectangle denotes the region used for fluorescence intensity profile analysis of AChRs and SV2. Intensity profiles were generated separately for each channel. Scale bar= 4 μm. Scale bar corresponds to the physical size of the expanded sample (not adjusted to expansion factor).

Article Snippet: Mouse anti-SV2 IgG antibody (1:50 dilution) , Developmental Studies Hybridoma Bank , Cat#2315387; RRID: AB-2315387.

Techniques: Staining, Immunolabeling, Stripping Membranes, Labeling, Fluorescence, Generated

Journal: Disease Models & Mechanisms

Article Title: Reduction of oxidative stress suppresses poly-GR-mediated toxicity in zebrafish embryos

doi: 10.1242/dmm.049092

Figure Lengend Snippet: Injection of ATG poly-GR evokes apoptosis and aberrant motor neuron axon morphology in 1-4-dpf zebrafish embryos. (A) Maximum projection of z -stack images of the SecA5 fluorescent reporter line (green) embryos 48 h after injection with 10 pg ATG poly-GR, 10 pg TAG poly-GR or 400 pg mCherry only. (B) Quantification of z -stack images of the SecA5 fluorescent reporter line embryos after injection with 10 pg ATG poly-GR, 10 pg TAG poly-GR or 400 pg mCherry only at 1-4 dpf. N =minimum of ten fish per group (mean±s.e.m.). P <0.0001 (Kruskal–Wallis test). * P <0.05, *** P <0.0001 (Dunn's post-hoc multiple comparison test), differences of ATG poly-GR-injected fish compared to 10 pg TAG poly-GR and mCherry only at 1-4 dpf. (C) Synaptic vesicle (SV2) in combination with α-bungarotoxin (BTX) staining demonstrates aberrant axonal protrusions in the tail of 2-dpf wild-type AB embryos after injection with 10 pg ATG poly-GR compared to 10 pg TAG poly-GR and 400 pg mCherry only. n =10 per group (mean±s.e.m.). (D) Fluorescence of SV2 staining was measured for five fish/group and three neurites per fish, and was significantly reduced in 10 pg ATG poly-GR-injected embryos. P <0.0001 (one-tailed t -test with Welch's correction). Scale bars: 100 µm.

Article Snippet: Subsequently, fish were stained for 30 min with 1 μg/ml α-bungarotoxin-TRITC (Invitrogen), washed with PBS and incubated overnight at 4°C with the anti-mouse IgG SV2 antibody (1:200, AB231587, Developmental Studies Hybridoma Bank, University of Iowa).

Techniques: Injection, Comparison, Staining, Fluorescence, One-tailed Test